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Human Erythroferrone (ERFE) ELISA Kit Human Erythroferrone (ERFE) ELISA Kit

Categories Human ELISA Kit
Place of Origin: China
Brand Name: DLdevelop
Certification: CE, ISO9001:2015
MOQ: 48T
Price: Negotiation
Packaging Details: Carton Box
Delivery Time: 1-2 Working Days
Payment Terms: TT
Supply Ability: Abundant Supply
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    Human Erythroferrone (ERFE) ELISA Kit Human Erythroferrone (ERFE) ELISA Kit

    Human Erythroferrone (ERFE) ELISA Kit

    0

    two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

    Traditional ELISA KitReady-to-Use ELISA KIT
    Product name:Human Erythroferrone (ERFE) ELISA Kit
    Method:Sandwich
    Synonyms:

    FAM132B; C1QTNF15; CTRP15; Myonectin; Family With Sequence Similarity 132, Member B; Complement C1q tumor necrosis factor-related protein 15

    Detection range:0.156-10ng/mL
    Reactivity:ERFE
    Size:96T/48T
    Quality guarantee period:for 12 months
    Catalog number:DL-ERFE-Hu (traditional)DLR-ERFE-Hu (ready-to-use)
    Assay length1-4.5Hours1-3.5Hours
    Advantages:
    • Competitive price.
    • High sensitivity.
    • High stability.
    • 12 months shelf life.
    • Pre-diluted Detection Reagent A and B
    • Reduction in the number of steps when conducting the test
    • All the reagents can be stored at 4℃
    • Faster reaction compare to other brands
    • 12 months shelf life
    Instruction Manual
    1.Overview:

    Other names:FAM132B; C1QTNF15; CTRP15; Myonectin; Family With Sequence Similarity 132, Member B; Complement C1q tumor necrosis factor-related protein 15

    Function: Iron-regulatory hormone that acts as an erythroid regulator after hemorrhage: produced by erythroblasts following blood loss and mediates suppression of hepcidin (HAMP) expression in the liver, thereby promoting increased iron absorption and mobilization from stores. Promotes lipid uptake into adipocytes and hepatocytes via transcriptional up-regulation of genes involved in fatty acid uptake.

    Sequence:

    50 MAPARRPAGA RLLLVYAGLL AAAAAGLGSP EPGAPSRSRA RREPPPGNEL 100 PRGPGESRAG PAARPPEPTA ERAHSVDPRD AWMLFVRQSD KGVNGKKRSR 150 GKAKKLKFGL PGPPGPPGPQ GPPGPIIPPE ALLKEFQLLL KGAVRQRERA 200 EPEPCTCGPA GPVAASLAPV SATAGEDDDD VVGDVLALLA APLAPGPRAP 250 RVEAAFLCRL RRDALVERRA LHELGVYYLP DAEGAFRRGP GLNLTSGQYR 300 APVAGFYALA ATLHVALGEP PRRGPPRPRD HLRLLICIQS RCQRNASLEA 350 IMGLESSSEL FTISVNGVLY LQMGQWTSVF LDNASGCSLT VRSGSHFSAVLLGV

    You can reference link of the kit as following
    http://www.dldevelop.com/Research-reagent/dl-erfe-hu.html
    http://www.dldevelop.com/uploadfile/data/DL-ERFE-Hu.pdf
    Introduction
    ItemStandardTest
    Description

    The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ERFE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

    Conform
    IdentificationColorimetricPositive
    CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
    Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
    Plate sealer for 96 wells 2Plate sealer for 96 wells 2
    Standard 2Standard 2
    Diluents buffer 1×45mLStandard Diluent 1×20mL
    Detection Reagent A 1×120LDetection Solution A 1×12mL
    Detection Reagent B 1×120LDetection Solution B 1×12mL
    TMB Substrate 1×9mLTMB Substrate 1×9mL
    Stop Solution 1×6mLStop Solution 1×6mL
    Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
    Instruction manual 1Instruction manual 1

    Test principle

    The microtiter plate provided in this kit has been pre-coated with an antibody specific to ERFE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ERFE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

    After TMB substrate solution is added, only those wells that contain ERFE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-sub







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