Technical Grade Bone Peptone with 14.5% Nitrogen Content Derived from Bovine Bone for Culture Media
Specialty Bone Peptone — Technical Grade Nitrogen Substrate for Professional Culture Media
Product Description
Specialty Bone Peptone is a technical-grade nitrogen substrate derived from fresh bovine bone through controlled enzymatic digestion. This product serves as a foundational nutrient component for industrial fermentation processes, professional microbiological media preparation, and research applications. The manufacturing process focuses on maintaining consistent nitrogen availability and physical characteristics suitable for routine laboratory and production use.
Raw Material and Production
| Parameter | Details |
|---|---|
| Raw Material | Fresh bovine bone from inspected sources |
| Processing Method | Thermal treatment, enzymatic hydrolysis, separation, filtration, spray drying |
| Production Standard | Manufactured under controlled conditions with in-process monitoring |
| Reference Document | Chinese Biological Products Raw Material Control Standard (2000) |
Key Characteristics
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Nitrogen Content – Total nitrogen ≥14.5% with amino nitrogen fraction supporting microbial metabolism
-
Solubility – Complete dissolution in water; solutions remain clear after heating and cooling
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Residue Profile – Controlled ash and chloride content for minimal media interference
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Physical Form – Free-flowing powder suitable for blending and formulation
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Batch Consistency – Regular production monitoring ensures reproducible performance
Technical Specifications
| Parameter | Test Method | Unit | Specification | Typical Value |
|---|---|---|---|---|
| Total Nitrogen | Kjeldahl | % | ≥ 14.5 | 15.2 |
| Amino Nitrogen | Formol titration | % | ≥ 2.5 | 3.1 |
| Sodium Chloride | Titration | % | ≤ 5.0 | 2.6 |
| Loss on Drying | 105°C, constant weight | % | ≤ 5.0 | 4.5 |
| Ash Content | 600°C | % | ≤ 5.0 | 3.6 |
| pH (2% solution) | Potentiometric | — | 5.0 – 7.0 | 5.4 |
| Appearance | Visual | — | Yellow powder | Conforms |
| Solution Clarity | 2% solution, boiled | — | Clear, no precipitate | Conforms |
| Alkaline Test | pH 8-9, 121°C, 20 min | — | No precipitate | Conforms |
| Nitrite | Colorimetric | — | Not detectable | Conforms |
Microbiological Test Results
| Test | Organisms | Expected | Result |
|---|---|---|---|
| Hydrogen Sulfide | S. Typhi / Shigella | + / – | Compliant |
| Indole | E. coli / S. Typhi | + / – | Compliant |
| Voges-Proskauer | E. aerogenes / E. coli | + / – | Compliant |
| Fermentation | E. coli / S. Typhi | No acid | Compliant |
| Hemolysis | S. pneumoniae / β-streptococcus | α / β | Maintained |
| Pigment | P. aeruginosa / S. aureus | Green/Gold | Maintained |
| Growth Support | E. coli / S. aureus | ≥1.0mm, smooth | Compliant |
| Sensitivity | β-streptococcus / Gram-positive bacillus | 10⁻⁴ / 10⁻⁵ | Compliant |
Applications
| Area | Use |
|---|---|
| Industrial Fermentation | Enzyme, antibiotic, organic acid production |
| Media Manufacturing | Dehydrated culture media, liquid media |
| Pharmaceutical Processing | Bacterial biomass, vaccine intermediates |
| Laboratory Research | Microbial cultivation, media development |
Packaging and Storage
| Item | Specification |
|---|---|
| Packaging | 20 kg net weight, polyethylene liner with outer bag |
| Storage | Cool, dry, ventilated area; protect from moisture |
| Shelf Life | 24 months from manufacture date |
| Handling | Keep sealed when not in use |
Contact our technical team for specific application guidance.
Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.