Agrose Magnetic Beads for Protein Purification - 30-150 μm Size, 10% Volume Ratio, 25-30 mg/mL IgG Capacity
Price:
Negotiation
MOQ:
5 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Agrose Magnetic Beads for Protein Purification
Product Overview
BeaverBeads™ Protein A and Protein G magnetic beads are superparamagnetic particles covalently coated with recombinant Protein A or Protein G Fc binding domains. These specialized beads enable efficient purification of monoclonal and polyclonal antibodies from various biological samples including cell lysate, blood plasma, ascites, and tissue culture supernatant. The product supports repeated use and is suitable for antibody immobilization studies.
Technical Specifications
| Parameter | Specification |
|---|---|
| Bead Size | 30-150 μm |
| Beads Concentration | 10% (v/v) |
| Antibody Binding Capacity | 25-30 mg Human IgG/mL Gel |
| Storage Temperature | 2-8°C |
| Shelf Life | 12 months |
| Binding/Washing Buffer | PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO₄, 0.1% Tween-20 |
| Elution Buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
| Neutralization Buffer | 1.0 M Tris-HCl, pH 9.0 |
| Storage Buffer | PBST (includes 0.05% NaN₃) |
Key Features
- High antibody binding capacity with ultra-low non-specific adsorption
- Time-saving, simple and mild purification process
- Exceptional product stability and reusability
- Near-neutral antibody elution system compatibility
- Minimal protein A ligand off rate
Technical Support & FAQs
How to improve antibody elution efficiency?
For high-affinity antibodies with low elution efficiency, optimize by reducing elution buffer pH (1.9-2.5), increasing ionic strength (2-3 M MgCl₂), or extending elution time. Note that antibodies may aggregate under low pH conditions - immediately neutralize with alkaline buffers (Tris, HEPES, etc.).
How to prevent magnetic bead adhesion to tube walls?
Use low-adsorption consumables and add 0.01%-0.1% (v/v) nonionic detergent (NP-40, Tween-20, or Triton X-100) to buffer solutions to minimize bead adhesion.
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Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.
Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine