Magrose Protein G Magnetic Beads 30-150 μm for Antibody Purification with 25-30 mg/mL IgG Binding Capacity
Price:
Negotiation
MOQ:
5 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Product Overview
Magrose Protein G magnetic beads provide efficient antibody purification with 30-150 μm bead size and 10% concentration. These magnetic beads enable rapid antibody adsorption within 15 minutes and complete purification within 30 minutes, suitable for plasma, ascites, and tissue culture supernatant samples.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Magrose Protein G |
| Bead Size | 30-150 μm |
| Beads Concentration | 10% (v/v) |
| Ligand | Protein G |
| Medium | Magrose |
| Antibody Bonding Capacity | 25-30 mg Human IgG/mL Gel |
| Storage Temperature | 2-8°C |
| Shelf Life | 12 months |
Key Features
- High antibody binding capacity with ultra-low non-specific adsorption
- Rapid processing: 15-minute adsorption, 30-minute purification
- Excellent product stability and reusability
- Near-neutral antibody elution system for better preservation
- Very low protein G ligand off rate
Buffer Solutions
Binding/Washing Buffer
PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO₄, 0.1% Tween-20
Elution Buffer
100 mM Gly, 0.1% Tween-20, pH 2.5
Neutralization Buffer
1.0 M Tris-HCl, pH 9.0
Storage Buffer
PBST (includes 0.05% NaN₃)
Frequently Asked Questions
How to improve antibody binding efficiency with magnetic beads?
Binding efficiency depends on antibody origin and subtype. For antibodies with low Protein G affinity, extend incubation time (30-120 min), increase buffer pH (8-9), or reduce ionic strength (25-100 mM NaCl). Consider using Protein A or Protein A/G ligands for higher affinity targets.
How to prevent magnetic bead adhesion to tube walls?
Use low-adsorption consumables and add 0.01-0.1% nonionic detergent (NP-40, Tween-20, or Triton X-100) to buffer solutions to minimize bead adhesion.
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Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.
Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine