PP PET Vacuum Blood Collection Tube 2ml-10ml Volume
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A vacuum blood colletion tube blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. vacuum blood colletion tube tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing. Tubes are available with a safety-engineered stopper, with a variety of labeling options and draw volumes. The color of the top indicates the additives in the vial.
Types of tubes:
vacuum blood colletion tube tubes may contain additional substances that preserve blood for processing in a medical laboratory. Using the wrong tube may make the blood sample unusable for the intended purpose. These additives are typically thin film coatings applied using an ultrasonic nozzle.
The additives may include anticoagulants (EDTA, sodium citrate, heparin) or a gel with density between those of blood cells and blood plasma. Additionally, some tubes contain additives that preserve certain components of or substances within the blood, such as glucose. When a tube is centrifuged, the materials within are separated by density, with the blood cells sinking to the bottom and the plasma or serum accumulating at the top. Tubes containing gel can be easily handled and transported after centrifugation without the blood cells and serum mixing.
The term order of draw refers to the sequence in which tubes should be filled. The needle which pierces the tubes can carry additives from one tube into the next, so the sequence is standardized so that any cross-contamination of additives will not affect laboratory results
| Tube cap color or type | Additive | Usage and comments |
|---|---|---|
| Blood culture bottle | Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms | Usually drawn first for minimal risk of contamination.[8] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms.[9] |
| Light blue | Sodium citrate (anticoagulant) | Coagulation tests such as prothrombin time (PT) and partial thromboplastin time (PTT) and thrombin time (TT). Tube must be filled 100%. |
| Plain red | No additive | Serum: Total complement activity, cryoglobulins |
| Gold (sometimes red and grey "tiger top"[10]) | Clot activator and serum separating gel[11] | Serum-separating tube: Tube inversions promote clotting. Most chemistry, endocrine and serology tests, including hepatitis and HIV. |
| Dark green | Sodium heparin (anticoagulant) | Chromosome testing, HLA typing, ammonia, lactate |
| Mint green | Lithium heparin (anticoagulant) | Plasma. Tube inversions prevent clotting |
| Lavender ("purple") | EDTA (chelator / anticoagulant) | Whole blood: CBC, ESR, Coombs test, platelet antibodies, flow cytometry, blood levels of tacrolimus and cyclosporin |
| Pink | EDTA (chelator / anticoagulant) | Blood typing and cross-matching, direct Coombs test, HIV viral load |
| Royal blue | EDTA (chelator / anticoagulant) | Trace elements, heavy metals, most drug levels, toxicology |
| Tan | EDTA (chelator / anticoagulant) | Lead |
| Gray |
|
Glucose, lactate[13] |
| Yellow | Acid-citrate-dextrose A (anticoagulant) | Tissue typing, DNA studies, HIV cultures |
| Pearl ("white") | Separating gel and (K2)EDTA | PCR for adenovirus, toxoplasma and HHV-6 |
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