N-Hydroxy Succinimide Nanoparticle NHS Activated Magnetic Beads 30-150 μm Size with 20% Ethanol Solution for Biomolecule Coupling
N-Hydroxy Succinimide Nanoparticle magnetic beads with 30-150 μm particle size and 20% (v/v) ethanol preservation solution. Ready-to-use preactivated beads for covalent coupling with biomolecules containing primary amine groups.
BeaverBeads™ Mag NHS and BeaverBeads™ Magrose NHS are spherical magnetic beads modified with NHS groups that form stable peptide bonds with proteins and other molecules containing primary amine groups. These beads are ideal for affinity purification and assay detection of antibodies, antigens, and various biomolecules.
| Parameter | Specification |
|---|---|
| Mean Particle Size | 30-150 μm |
| Magnetic Core | Fe3O4 |
| Shell Material | Agarose |
| Magnetic Properties | Superparamagnetic |
| Ligand Content | ~50 μM/mL gel |
| Binding Capacity | 20-30 mg rabbit IgG/mL beads |
| Preservation Solution | 20% (v/v) ethanol |
| Operation Mode | Automated/Manual |
- Simple operation - No activation required, direct covalent coupling with biological ligands
- High efficiency - Up to 90% bio-ligand coupling efficiency with enhanced binding capacity
- Rapid processing - Complete bio-ligand coupling in 1-2 hours
- Mild conditions - Room temperature or 4°C coupling with pH range 5-9
- Exceptional stability - Forms stable amide bonds preventing ligand detachment
- Superior biocompatibility - Reduced non-specific adsorption for cleaner results
These preactivated magnetic beads eliminate the need for traditional activation steps using EDC/NHS or glutaraldehyde. Simply mix the protein solution containing primary amino groups with Mag NHS beads at room temperature for 1-2 hours to achieve efficient ligand attachment. The superparamagnetic properties ensure easy separation and handling in both automated and manual workflows.
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