2 μm Polymer NHS Magbeads Preactivated 10 mg/mL 50 mL - Superparamagnetic Nanoparticles for Protein Conjugation
Price:
Negotiation
MOQ:
1 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Polymer NHS Magbeads Preactivated - Superparamagnetic nanoparticles with N-Hydroxysuccinimide activated groups for covalent coupling with primary amines on biological ligands. These 2 μm beads offer simplified protein conjugation without requiring activation steps.
Product Overview
NHS magnetic beads feature superparamagnetic properties with activated N-Hydroxysuccinimide groups on the surface, enabling direct covalent binding with primary amines on target ligands through stable amide linkages. Compatible with various biomolecules including antigens, antibodies, and proteins.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Polymer NHS Magbeads Preactivated |
| Mean Particle Size | 2 μm |
| Magnetic Core | Fe3O4 |
| Shell Material | Polymer |
| Magnetic Properties | Superparamagnetism |
| Ligand Content | ~50 μM/mL gel |
| Binding Capacity | 20-30 mg rabbit IgG/mL beads |
| Concentration | 10 mg/mL |
| Volume | 50 mL |
| Preservation Fluid | DMAC |
| Operation Mode | Automated or Manual |
Key Advantages
- Simplified Protocol: Direct covalent coupling without EDC/NHS or glutaraldehyde activation steps
- High Efficiency: Bio-ligand coupling efficiency exceeding 90% with superior binding capacity
- Rapid Processing: Complete coupling in 1-2 hours at room temperature
- Mild Conditions: Compatible with pH 5-9 and temperatures from 4°C to room temperature
- Stable Bonding: Forms stable amide bonds preventing ligand dissociation
- Biocompatible: Reduced non-specific adsorption for cleaner results
Handling Instructions
Storage & Handling
- Store at 4°C and immediately reseal containers after sampling
- Prevent drying or freezing to maintain bead dispersion and binding activity
- Coupling must be performed in amino-free buffer solutions
Protein Detection Methods
Recommended assays include Thermo Scientific Pierce 660nm Protein Assay (Product No. 22660, 22662) or Micro BCA Protein Assay (Product No. 23235). Avoid 280nm wavelength detection due to NHS group interference.
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Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine