Calibrator L, Calibrator H: Tris buffer (0.15M, PH7.5) containing 0.5% BSA and 0.05% proclin300 was added with different amounts of VB12, the concentrations of VB12 were 250.0pg/mL and 900.0pg/mL, respectively.

Quality control product 1, quality control product 2: Tris buffer (0.15M, PH7.5) containing 0.5% BSA was added with different amounts of VB12, and the concentrations of VB12 were 250.0 pg/mL and 900.0 pg/mL, respectively.

Anti-Reagent A: 0.5M sodium hydroxide solution.

Anti-reagent B: ethanol solution containing 0.02% dithiothreitol.

Anti-reagent C: 0.3 μg/mL alkaline phosphatase (AP)-labeled vitamin B12 derivative (bovine source), 1.0 mg/mL magnetic particles conjugated with goat anti-FITC polyclonal antibody, containing 0.5% bovine serum albumin ( BSA) in Tris buffer (0.15M, pH 8.0), 0.05% proclin300.

Anti-reagent D: 0.5 μg/mL fluorescein isothiocyanate (FITC)-labeled mouse monoclonal anti-VB12 antibody, Tris buffer (0.15M, PH8.0) containing 0.5% BSA, 0.05% proclin300.
 

Note: Reagents of different batches of kits are not allowed to be used interchangeably.

Product picture