15 Min Malaria P.F/Pan Antigen Rapid Test Whole Blood Test Cassette

INTRODUCTION

Malaria is caused by a protozoan which invades human red blood cells. Malaria antigen (pf/pan) test paper is used to detect specific Plasmodium falciparum lactate dehydrogenase (pfLDH) and Plasmodium lactate dehydrogenase (panLDH) in whole blood samples.

INTENDED USE

Malaria P.f/Pan Rapid Test is a rapid chromatographic immunoassay for the qualitative detection of circulating antigens of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, Plasmodium malariae in whole blood.

For professional use only.

KEY PONITS

• High accuracy
• Easy to use
• Read in short time- 15 mins
• Storage at room temperature

TEST PROCEDURE

Allow the test device, specimen, buffer, and/or controls to equilibrate to room temperature (15-30°C) prior to testing.

1.Remove the test device from the foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed within one hour.

2.Place the test device on a clean and level surface. Transfer the specimen by a pipette or a disposable specimen pipette:

• To use a Pipette: Transfer 5 uL of whole blood to Well-S (S) of the test device, then add 3~4 full drops of buffer to Well-B (B), and start the timer. (See illustration ① below). Avoid trapping air bubbles in S.
• To use a Disposable Specimen Pipette: Hold the pipette vertically; draw the specimen up to the Fill Line as shown in illustration ① below. Transfer the specimen to S of the test device, then add 3~4 full drops of buffer to B and start the timer.Avoid trapping air bubbles in S.

3. Wait for the colored line(s) to appear. The result should be read at 15 minutes. Do not interpret the result after 20 minutes.

RESULT INTERPRETATION

POSITIVE:* Two or Three distinct colored lines appear.

P. falciparum or mixed malaria infection:one line appears in the control region, one line appears in Pan line region and one line appears in P.f line region.

P. falciparum infection: one line appears in the control region, and one line appears in P.f line region.

Non-falciparum Plasmodium species infection : one line appears in the control region and one line appears in Pan line region.

*NOTE: The color intensity of P.f or Pan test lines may vary depending on the concentration of antigens, viz., HRP-II or Aldolase present in the specimen.

NEGATIVE: Only one colored line appears in the control region.

INVALID: Control line fails to appear.

DETAILED PICTURE