Microprocessor Controller Stainless Steel LAI-D2 Anaerobic Workstation For Lab Cell Culture

Recommendations Before Entering the Transfer Chamber

• Make a list of the things you require to limit the amount of times you go into and out of the chamber. Too many times wastes gas and time and leads to oxygen contamination.
• Before bringing your samples or equipment into the anaerobic chambers, purge them of oxygen by bringing them into the transfer chamber. Then cycle the transfer chamber between vacuum and gas mix (95%N2/ 5% H2) no less than three times before you open the inner chamber. Make sure the chamber isn’t under the vacuum cycle when you open the door. Remember: Always have the inner door closed and sealed!
• Bring any required equipment into the chamber at least a night before you plan to begin an experiment.
• Purge or degas buffers/solutions for 30 minutes to an hour with an inert gas such as argon or nitrogen before you bring them in to the intake chamber.
• Sometimes the catalyst ‘stops working’ and the oxygen detector starts to sense oxygen. Don’t worry, just bake and renew your catalyst as recommended.
• If you are working with a stable enough protein requiring disulfide reduction, leave the protein to incubate anaerobically with the reductant. Then dialyze the reductant away in the chamber overnight. In place of dialysis, use a desalting spin column to quickly remove any residual reductant.
• If the protein is not stable enough for overnight dialysis in the chamber, briefly purge it with an inert gas, and then equilibrate in the chamber 30 minutes to an hour prior to disulfide reduction.

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