Cfx96 Bio-Rad Connect Real Time Pcr Detection System In Gene Expression Level Analysis Fields
CFX Connect Real-Time PCR Detection System
The CFX Connect Real-Time PCR System provides excellent thermal performance for two-target multiplex PCR and routine detection of single fluorophore experiments.
The CFX Connect Real-Time PCR Detection System offers two-target analysis, excellent thermal cycler specifications, and the same reliable performance as the CFX96 Touch Real-Time PCR Detection System. The system incorporates innovative optical technologies with powerful software to provide maximal reliability and efficiency for all your real-time PCR needs.
Easy startup — obtain great results right away with factory-calibrated dyes, quick setup, and intuitive software
Effortless optimization — save time and reduce costs by optimizing assays in a single run using the thermal gradient
Powerful data analysis tools — quickly and accurately validate and analyze data with the advanced analysis modules of CFX Maestro Software
Accelerated publication submission — include MIQE annotations and generate RDML files using Biogazelle's qbase+ Software
It can be used in a variety of research fields such as nucleic acid quantification, gene expression level analysis, gene mutation detection, GMO detection and product specificity analysis.
| Chassis | CFX Connect |
| Maximum ramp rate, °C/sec | 5 |
| Average ramp rate, °C/sec | 3.3 |
| Heating and cooling method | Peltier |
| Lid, °C | Heats up to 105 |
| Temperature | |
| Range, °C | 0–100 |
| Accuracy, °C | ±0.2 of programmed target at 90°C |
| Uniformity, °C | ±0.4 well-to-well within 10 sec of arrival at 90°C |
| Gradient | |
| Operational range, °C | 30–100 |
| Programmable span, °C | 1–24 |
| Optical Detection | |
| Excitation | 3 filtered LEDs |
| Detection | 3 filtered photodiodes |
| Range of excitation/emission wavelengths, nm | 450–580 |
| Sensitivity | Detects 1 copy of target sequence in human genomic DNA |
| Dynamic range | 10 orders of magnitude |
| Scan Time | |
| All channels, sec | 12 |
| FAM/SYBR® Green only, sec | 3 |
| Software | |
| Operating systems | Windows 7, Windows 8, Windows 10 |
| Multiplex analysis | Up to 2 targets per well |
| System | |
| Licensed for real-time PCR | Yes |
| Sample capacity, wells | 96 |
| Sample size, µl | 1–50 (10–25 recommended) |
| Communication interface | USB 2.0 |
| Electrical approvals | IEC, CE |
| Dimensions (W x L x H), cm/in | 33 x 46 x 36/13 x 18 x 14 |
| Weight, kg/lb | 21/47 |
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