Quantitative Nt Probnp IVD Kit Serum / Blood Ce Test Kit

Blood samples were drawn between 8.00 am and 10.00 am within 24 hours of admission. Samples were collected in prechilled tubes containing EDTA, immediately placed on ice, and promptly centrifuged at 4°C. After separation, plasma was stored at −80°C. NT-proBNP measurements were done using an ELISA—a two step sandwich assay with streptavidin coated microtitre plates.14 This assay does not require sample extraction and there is no detectable cross reactivity with ANP, NT-proANP, BNP, or urodilatin. The interassay and intra-assay variances were 10% and 3%, and recovery was between 104% and 112%. In patients with an LVEF of > 50%, 85% of subjects had an NT-proBNP value below 357 pmol/l (1n(357) = 5.9). This value was used as the upper normal limit in the analyses of sensitivity and specificity. It should be stressed that 357 pmol/l is not a true normal value but a cut off value derived from the present hospital inpatient population.

Main components

The kit consists of test card, buffer and ID chip (optional). Among them:

1. Test card: composed of reagent strip and plastic box. The main components of the reagent strip were: nitrocellulose membrane, absorbent paper, PVC plate and label pad; the strip was coated with mouse anti-human NT-proBNP monoclonal antibody (0.28 μ g-1.12 μ G on each reagent strip), rabbit IgG (0.20 μ g-1.50 μ g per sample), mouse anti human NT-proBNP monoclonal antibody (0.03 μ g-0.10 μ g per sample) and fluorescein The rabbit IgG (0.20 μ g-1.50 μ g) was recorded by the cursor.

2. Buffer: tris (50mmol / L).

Main Package