Neutralizing Antibody Combo Rapid Test Kit For SARS-CoV-2

Test principle

Based on the principle of Immunofluorescence analysis, SARS-CoV-2 Neutralizing Antibody Assay Kit by Immunofluorescence Chromatography Method was used to immobilized ACE-2 protein (T line) and goat anti-chicken IgY Antibody (C line) using nitrocellulose membrane (NC) as solid phase carrier. Fluorescent microspheres were conjugated to recombinant SARS-CoV-2 S-RBD antigen in the fluorescent labeling pad.

When the buffer solution was added to the sample well, the S-RBD-labeled fluorescent microspheres in the labeling pad were pushed to bind to the corresponding immobilized ACE-2 protein on the NC membrane, and the fluorescent signal could be detected at the marking position. If there is a neutralizing antibody in the sample, it will bind to the labeled S-RBD antigen when passing through the labeling pad. The neutralizing antibody can prevent the binding of S-RBD to ACE-2, resulting in a decrease in the signal value, and he T-line signal value is negatively correlated with the neutralizing antibody content.