Bone Peptone (Biological Grade) — High Nitrogen Content (≥14.5%), Complete Solubility & Clarity, Low Ash Content (≤5.0%) for Microbial Fermentation
Bone Peptone (Biological Grade) — Premium Nitrogen Source for Microbial Fermentation
Product Overview
Bone Peptone is a high-performance nitrogen source manufactured from fresh bovine bone through controlled enzymatic hydrolysis. Designed for biological pharmaceutical production, microbiological culture media, and fermentation processes, this product delivers consistent nutrient profiles and exceptional clarity for reliable microbial growth.
Manufacturing Process
| Stage | Description |
|---|---|
| Raw Material Preparation | Fresh bovine bone inspection, washing, and sterilization |
| Enzymatic Hydrolysis | Controlled pancreatic enzyme digestion at low temperature |
| Purification | Separation, decolorization, deodorization, and refined filtration |
| Concentration & Drying | Ultrafiltration, concentration, sterilization, and spray drying |
| Quality Control | In-process testing, metal detection, and final inspection |
Key Performance Characteristics
| Parameter | Performance |
|---|---|
| Nitrogen Content | High total nitrogen (≥14.5%) with available amino nitrogen (≥2.5%) for optimal microbial utilization |
| Solubility & Clarity | Complete solubility in water; solutions remain clear and precipitate-free after heating and pH adjustment |
| Purity | Low ash content minimizes interference in sensitive applications |
| Batch Consistency | Controlled production ensures reproducible performance across batches |
Quality Specifications
| Test Parameter | Unit | Specification | Typical Value |
|---|---|---|---|
| Total Nitrogen | % | ≥ 14.5 | 15.2 |
| Amino Nitrogen | % | ≥ 2.5 | 3.1 |
| Sodium Chloride | % | ≤ 5.0 | 2.56 |
| Loss on Drying | % | ≤ 5.0 | 4.5 |
| Ash Content | % | ≤ 5.0 | 3.62 |
| pH (2% solution) | — | 5.0 – 7.0 | 5.4 |
| Appearance | — | Yellow powder | Yellow powder |
| Solution Clarity | — | Clear, no precipitate | Conforms |
| Alkaline Precipitation | — | No precipitate | Conforms |
Microbiological Performance Data
| Test | Test Strains | Acceptance Criteria | Result |
|---|---|---|---|
| Hydrogen Sulfide Production | S. typhi / Shigella | + / – reaction | Compliant |
| Indole Production | E. coli / S. typhi | + / – reaction | Compliant |
| Voges-Proskauer Reaction | E. aerogenes / E. coli | + / – reaction | Compliant |
| Carbohydrate Fermentation | E. coli / S. typhi | No acid production | Compliant |
| Hemolytic Reaction | S. pneumoniae / β-streptococcus | α / β hemolysis | Stable through passages |
| Pigment Production | P. aeruginosa / S. aureus | Green / Golden | Stable through passages |
| Growth Support | E. coli / S. aureus | Colony size ≥1.0mm, >95% smooth colonies | Conforms |
| Sensitivity | Streptococcus / Gram-positive bacillus | ≥10⁻⁴ / ≥10⁻⁵ dilution | Conforms |
Applications
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Industrial Fermentation: Antibiotics, enzymes, amino acids, and organic acids production
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Microbiological Media: Dehydrated culture media, liquid media, and diagnostic formulations
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Biopharmaceutical Manufacturing: Vaccine production, cell culture, and fermentation processes
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Research Applications: Laboratory-scale fermentation and microbiological studies
Packaging and Storage Information
| Parameter | Details |
|---|---|
| Packaging | 20 kg net weight, inner polyethylene liner with outer woven bag or paper drum |
| Storage Conditions | Store in cool, dry, well-ventilated area away from direct sunlight |
| Shelf Life | 24 months when stored under recommended conditions |
| Handling | Keep sealed when not in use; protect from moisture and contamination |
Regulatory Compliance
-
Quality Standard: Q/SCSH 001-2025 (Enterprise Standard)
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Reference Standard: Chinese Biological Products Main Raw and Auxiliary Materials Quality Control Standard (2000 Edition)
-
Test Methods: GB 5009 series (National Food Safety Standards) and referenced pharmacopoeia methods
Technical data represents typical analysis. For specific application requirements or customized specifications, please contact our technical support team.
Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.