NSE Tumor Marker Rapid Quantitative Test Kit 20T WWHS FIA POCT Assay

Neuron specific enolase (NSE) which can also be called gamma-enolase (ENO-2) or 2-phospho-D-glycerate

hydrolase, is an isoenzyme belonging to the enolase family that consists up of α, β and γ subunits

either has a homo or heterodimer. NSE is a 78 kD gamma-homodimer and the majority of this enolase-

isoenzyme can be found in neuroendocrine and neuronal tissues. It is essentially a glycolytic enzyme

that is able to catalyse the conversion of 2-phosphoglycerate into phosphoenolpyruvate. The

biological half-life of neuron specific enolase in body fluids is about 24 hours and due to the

organ-specificity and concentrations of NSE in serum or cerebrospinal fluid (CSF), elevated levels

have been identified in diseases which result in relative rapid neuronal destruction.

Other WWHS Assay Items

Test Method

1. Please thoroughly read the specification before test. Frozen test card and sample should be placed at room temperature (15-30) ℃ for at least 30min before use.
2. Start NIR-1000 dry fluoroimmunoassay analyser and verify quality control according to the specification.
3. The quality of reagent is controlled using internal quality control product (not provided) of the company and result should be controllable.
4. Take out the test card from the aluminum foil bag and use it within 15min
5. Place the test card on a clean horizontal table top and label it.
6. Take 10μL of sample and add it into HbA1c sample buffer (1.00mL). Then, mix the solution evenly, take 100μL of the solution and add it into the well.
7. Insert the test card into NIR-1000 dry fluoroimmunoassay analyser, keep time for 10min and press 【Instant test】. The analyser will judge and read the test result automatically. Or press 【Fixed time test】 to keep time for 10min automatically. The analyser will judge and read the test result automatically and display it in the screen.

Applicable Instrument

WWHS NIR-1000 dry fluoroimmunoassay analyser