[Main Ingredients]

[Sample Requirements]

1. Serum and EDTA·Na₂ anticoagulant plasma and whole blood, EDTA·K₂ anticoagulant plasma and whole blood, sodium citrate anticoagulant plasma and whole blood can be used.
2. Collect venous blood using conventional laboratory method and avoid hemolysis in the treatment process.
3. Clinical samples should be tested at room temperature (15-30)℃ in 4h after collected. Whole blood specimens can be stored at (2-8)℃ for 24h and should not be frozen; serum or plasma specimens can be stored at (2-8)℃ for 7 days and at -20ºC for 30 days.
4. The sample must be re-warmed to room temperature (15-30)℃ before test. Frozen samples should be completely melted, re-warmed and mixed before use and should not be frozen repeatedly.
5. Please do not test samples of severe hemolysis, severe lipoidemia and icterus.

[Test Method]

1. Please thoroughly read the specification before test. Frozen test card and sample should be placed at room temperature (15-30)℃ for at least 30min before use.
2. Start NIR-1000 dry fluoroimmunoassay analyser and verify quality control according to the specification. (Note: Reagent has been calibrated in advance and calibration curve parameters of each batch of reagents have been stored in the information card. Insert the information card before use and carry out test without re-calibration after passing quality inspection; otherwise, identify the cause before test.)
3. Take out the test card from the aluminum foil bag and use it within 15min
4. Place the test card on a clean horizontal table top and label it.
5. Serum, plasma or whole blood specimen: Take 100μL of sample and add it into 300μL of buffer solution (1:3). Then, mix the solution evenly, take 100μL of the solution and add it into the test card well.
6. Insert the test card into NIR-1000 dry fluoroimmunoassay analyser and press “Timing test” to keep time for 12min automatically. The analyser will judge and read the test result automatically and display it in the screen. Or insert the test card into the analyser after 12min and press “Instant test”, the instrument will judge and read the result automatically.