2μm Protein A Antibody Purification Kit with 30 mg/mL Capacity for Monoclonal and Polyclonal Antibody Isolation
Price:
Negotiation
MOQ:
1 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Protein A Antibody Purification Kit Overview
BeaverBeads™ Protein A and Protein G are superparamagnetic beads covalently coated with recombinant proteins containing Fc binding domains for efficient purification of monoclonal or polyclonal antibodies from various sources including cell lysate, blood plasma, ascites, and tissue culture supernatant.
Product Specifications
| Component | Quantity |
|---|---|
| Protein A (or A/G) for Antibody Purification | 1 mL |
| Antibody Binding Buffer | 100 mL |
| Antibody Elution Buffer I (pH 4.5) | 50 mL |
| Antibody Elution Buffer II (pH 2.0) | 50 mL |
| Antibody Neutralization Buffer | 10 mL |
| Beads Washing Buffer | 10 mL |
| Beads Storage Buffer | 10 mL |
| Beads Regeneration Buffer | 10 mL |
| Storage Conditions | 2~8°C for long-term preservation |
| Shelf Life | 2 years |
Key Features & Benefits
Magnetic separation eliminates need for centrifugation, reducing processing time significantly
One-step solution reduces antibody absorption to 10 minutes and purification to 30 minutes
Achieves >90% antibody purity with minimal protein loss
Prevents mechanical shearing damage to active proteins during processing
2 μm bead size with 30 mg/mL binding capacity for optimal performance
Frequently Asked Questions
How to improve antibody binding efficiency with magnetic beads?
Binding efficiency depends on antibody origin and subtype. For antibodies with low Protein A affinity, extend incubation time (30-120 min), increase buffer pH (8-9), reduce ionic strength (25-100 mM NaCl), or select higher affinity ligands like Protein G or Protein A/G.
How to enhance antibody elution efficiency?
For high-affinity antibodies, improve elution by reducing buffer pH (1.9-2.5), increasing ionic strength (2-3 M MgCl2), or extending elution time. Note that antibodies may aggregate under low pH conditions - adjust to neutral immediately with alkaline buffers like Tris or HEPES.
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Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine