Magrose Protein G Magnetic Beads 30-150 μm 10% Volume Ratio for Antibody Purification 25-30 mg/mL Capacity
Price:
Negotiation
MOQ:
5 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Magrose Protein G Magnetic Beads - Complete antibody purification in 30 minutes with >90% purity using 30-150 μm beads at 10% volume ratio.
Product Overview
These micron magnetic beads enable rapid antibody purification, completing the adsorption process within 15 minutes and full purification within 30 minutes. The one-step solution yields antibodies with >90% purity, suitable for antibody fixation and related research applications.
Technical Specifications
| Parameter | Specification |
|---|---|
| Bead Size | 30-150 μm |
| Beads Concentration | 10% (v/v) |
| Ligand | Protein A |
| Medium | Magrose |
| Antibody Bonding Quantity | 25-30 mg Human IgG/mL Gel |
| Storage Temperature | 2-8°C |
| Shelf Life | 12 months |
Key Features
- High antibody binding capacity (25-30 mg/mL) with ultra-low non-specific adsorption
- Rapid processing: 15-minute adsorption, 30-minute complete purification
- Excellent product stability and reusability
- Neutral antibody elution system for better preservation
- Very low protein A ligand off rate
- Consistent >90% purity yield
Buffer Specifications
| Buffer Type | Composition |
|---|---|
| Binding/Washing Buffer | PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO₄, 0.1% Tween-20 |
| Elution Buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
| Neutralization Buffer | 1.0 M Tris-HCl, pH 9.0 |
| Storage Buffer | PBST (includes 0.05% NaN₃) |
Frequently Asked Questions
Q: How to avoid possible aggregation of magnetic beads during storage or use?
A: Store beads at 2-8°C and avoid contamination or drying. Magnetic beads aggregation at low pH elution buffer is normal and doesn't affect performance. Adding 0.1% nonionic detergent (NP-40, Tween-20 or Triton X-100) to buffers effectively prevents aggregation.
Q: Magnetic beads appear agglomeration during use?
A: Agglomeration typically occurs from prolonged magnetic field exposure. Use 2-minute ultrasonic water bath treatment to disperse beads, but avoid this method until after elution to prevent antibody loss.
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Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine