Strep-tag II Magnetic Agarose Beads 30-150 μm 10% Volume Ratio 5 mL for Protein Purification
Strep-tag II Magnetic Agarose Beads provide efficient purification of Strep(II)-tagged proteins using superparamagnetic technology. These 30-150 μm beads with 10% volume ratio offer rapid separation and high-purity protein isolation in a single step.
| Parameter | Specification |
|---|---|
| Bead Size | 30-150 μm |
| Ligand Density | ~6 mg Strep-Tactin / mL Gel |
| Protein Binding Capacity | ~7 mg Strep-tag II protein / mL Gel |
| Suspension Concentration | 10% (v/v) magnetic beads suspension |
| Binding Buffer | 50 mM Tris-HCl, pH 8.0 |
| Elution Buffer | 2.5 mM desthiobiotin in Binding Buffer |
| Storage Buffer | 1×PBS (0.1% Tween-20 + 0.05% NaN3) |
| Shelf Life | 2 years when stored at 2-8°C |
| Regeneration Buffer | 0.5 M NaOH or 1 mM HABA in Binding Buffer |
- High antibody binding capacity for efficient protein capture
- Time-saving magnetic separation process with gentle handling
- One-step purification achieving over 99% protein purity
- No tag removal required - maintains protein bioactivity
Note 1: Protein binding capacity varies based on target protein characteristics. Provided values are reference only.
Note 2: Product shipped in pure water for transportation. For long-term preservation, store in 20% ethanol solution.
The Strep-tag II system utilizes an eight-amino acid synthetic peptide (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that doesn't interfere with protein folding or biological activity. This enables isolation of functional proteins directly from crude cell lysates under gentle, physiological conditions.
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