Protein A Antibody Magnetic Beads Purification Kit - 2μm Size, 30 mg/mL Capacity, 5 mL Volume for Rapid Antibody Isolation
Price:
Negotiation
MOQ:
1 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Product Overview
BeaverBeads™ Protein A Antibody Purification Kit featuring 2μm magnetic beads with 30 mg/mL binding capacity in a 5 mL format. This complete purification system enables rapid antibody isolation from various biological samples with optimized buffer formulations.
Key Specifications
| Parameter | Specification |
|---|---|
| Bead Size | 2μm |
| Binding Capacity | 30 mg/mL |
| Kit Volume | 5 mL |
| Storage Temperature | 2-8°C |
| Shelf Life | 2 years |
Kit Components
| Component | Quantity |
|---|---|
| Protein A (or A/G) for Antibody Purification | 1 mL |
| Antibody Binding Buffer | 100 mL |
| Antibody Elution Buffer I (pH 4.5) | 50 mL |
| Antibody Elution Buffer II (pH 2.0) | 50 mL |
| Antibody Neutralization Buffer | 10 mL |
| Beads Washing Buffer | 10 mL |
| Beads Storage Buffer | 10 mL |
| Beads Regeneration Buffer | 10 mL |
Performance Advantages
- Magnetic separation eliminates centrifugation requirements, reducing processing time to 15-30 minutes
- Minimizes sample loss from repeated pipetting and fluid transfer
- Protects protein integrity by avoiding mechanical shear damage from centrifugation
- Reusable magnetic beads with regeneration capability for cost-effective operations
- Compatible with plasma, ascites, tissue culture supernatant, and other biological samples
Technical Guidance
How to improve antibody binding efficiency with magnetic beads?
Binding efficiency depends on antibody origin and subtype. For low-affinity antibodies, extend incubation time (30-120 minutes), increase binding buffer pH (8-9), reduce ionic strength (25-100 mM NaCl), or select alternative ligands (Protein G or Protein A/G).
How to enhance antibody elution efficiency?
For high-affinity antibodies requiring improved elution, reduce elution buffer pH (1.9-2.5), increase ionic strength (2-3 M MgCl2), or extend elution time. Immediately neutralize with alkaline buffers (Tris, HEPES) to prevent antibody aggregation.
Applications
Ideal for antibody purification from multiple sample types, antibody immobilization studies, and research requiring rapid, efficient protein isolation with minimal sample loss.
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Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.
Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine