Magrose Protein A Magnetic Beads 30-150 μm 10% Volume Ratio for Antibody Purification
Superparamagnetic beads with 30-150 μm size and 10% volume concentration for rapid antibody purification and immobilization studies.
BeaverBeads™ Protein A and Protein G are superparamagnetic beads covalently coated with recombinant Protein A or Protein G containing Fc binding domains. Designed for purification of monoclonal or polyclonal antibodies from cell lysate, blood plasma, ascites, and tissue culture supernatant, as well as antibody immobilization studies.
Skilled operators can complete antibody adsorption within 15 minutes and full antibody purification within 30 minutes. The product enables antibody fixation and related research applications.
| Parameter | Specification |
|---|---|
| Product Name | Magrose Protein A |
| Bead Size | 30-150 μm |
| Beads Concentration | 10% (v/v) |
| Ligand | Protein A |
| Medium | Magrose |
| Antibody Bonding Capacity | 25-30 mg Human IgG/mL Gel |
| Storage Temperature | 2-8°C |
| Shelf Life | 12 months |
| Buffer Type | Composition |
|---|---|
| Binding/Washing Buffer | PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO₄, 0.1% Tween-20 |
| Elution Buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
| Neutralization Buffer | 1.0 M Tris-HCl, pH 9.0 |
| Storage Buffer | PBST (includes 0.05% NaN₃) |
- High antibody binding capacity with ultra-low non-specific adsorption
- Time-saving operation with simple and mild processing conditions
- Excellent product stability for reliable performance
- Neutral antibody elution system for improved antibody integrity
- Very low Protein A ligand off rate for consistent results
- Reusable design for cost-effective applications
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