Agrose Protein A Magnetic Beads 30-150 μm 10% Volume Ratio for Antibody Purification 500 mL
Price:
Negotiation
MOQ:
5 mL / bottle
Delivery Time:
5-8 days
Brand:
BeaverBeads
Product Description
Agrose Protein A Magnetic Beads - Complete antibody purification in 30 minutes with >90% purity. 30-150 μm bead size with 10% volume concentration for efficient protein separation.
Product Overview
Agrose Protein A Magnetic Beads provide rapid antibody purification with exceptional efficiency. Skilled operators can complete antibody adsorption within 15 minutes and full purification within 30 minutes, achieving >90% purity. These reusable beads are ideal for purifying antibodies from plasma, ascites, tissue culture supernatant, and various biological samples.
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Magrose Protein A |
| Bead Size | 30-150 μm |
| Beads Concentration | 10% (v/v) |
| Ligand | Protein A |
| Medium | Magrose |
| Antibody Bonding Capacity | 25-30 mg Human IgG/mL Gel |
| Storage Temperature | 2-8°C |
| Shelf Life | 12 months |
Buffer Systems
| Buffer Type | Composition |
|---|---|
| Binding/Washing Buffer | PBST (pH 7.2-7.4): 137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2.0 mM KH₂PO₄, 0.1% Tween-20 |
| Elution Buffer | 100 mM Gly, 0.1% Tween-20, pH 2.5 |
| Neutralization Buffer | 1.0 M Tris-HCl, pH 9.0 |
| Storage Buffer | PBST (includes 0.05% NaN₃) |
Key Features
- High antibody binding capacity with ultra-low non-specific adsorption
- Time-saving operation with simple and mild processing conditions
- Exceptional product stability for reliable performance
- Near-neutral antibody elution system for protein preservation
- Very low Protein A ligand off rate for consistent results
- Reusable design for cost-effective purification workflows
Frequently Asked Questions
Q: How to improve antibody elution efficiency?
A: For high-affinity antibodies with low elution efficiency, reduce elution buffer pH (1.9-2.5), increase ionic strength (2-3 M MgCl₂), or extend elution time. Immediately neutralize with alkaline buffers (Tris, HEPES) to prevent antibody aggregation at low pH.
Q: How to prevent magnetic bead aggregation?
A: Store at 2-8°C and avoid contamination or drying. Add 0.1% nonionic detergent (NP-40, Tween-20, Triton X-100) to buffers. For aggregated beads in low pH, wash to neutral and use 2-minute ultrasonic water bath treatment.
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Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.
Company
BEAVER Biomedical Engineering Co., LTD.
Location
NE36 Nanopolis Suzhou, 99 Jinji Lake Avenue, Suzhou Industrial Park
Contact Person
Elaine