NHS Activated Magnetic Beads 10-30 μm with 20% Volume Ratio for Protein Conjugation and Immunoassays
NHS Activated Magnetic Beads with 10-30 μm particle size and 20% volume ratio are preactivated agarose magnetic nanoparticles designed for covalent coupling with primary amine-containing ligands. These superparamagnetic beads feature N-Hydroxysuccinimide surface groups for efficient protein conjugation without additional activation steps.
| Parameter | Specification |
|---|---|
| Mean Particle Size | 10-30 μm |
| Magnetic Core | Fe3O4 |
| Shell Material | Agarose |
| Magnetic Properties | Superparamagnetism |
| Ligand Content | ~50 μM/mL gel |
| Binding Capacity | 20-30 mg rabbit IgG/mL beads |
| Preservation Fluid | 20% (v/v) ethanol solution |
| Operation Mode | Automated or Manual |
- Simple operation - No activation required, direct covalent coupling with biological ligands
- High efficiency - Up to 90% coupling efficiency with enhanced binding capacity
- Rapid processing - Complete ligand coupling in 1-2 hours
- Mild conditions - Room temperature or 4°C coupling with pH 5-9 range
- Stable bonding - Forms stable amide bonds to prevent ligand detachment
- Excellent biocompatibility - Reduced non-specific adsorption
Magnetic beads are moisture-sensitive. Immediately reseal containers after sampling and store at 4°C with proper sealing.
Protein stabilizers (BSA, gelatin) inhibit antibody binding. Ensure no primary amine-containing stabilizers are present during coupling procedures. Remove primary amine materials using dialysis or desalination methods before coupling.
Get in Touch
Have questions about our products or want to discuss a custom order? Our team is ready to help you.