Circovirus Type 2 Porcine Test Kit Antibody ELISA Test Kit For Pig 192 Wells/Kit GMP

Porcine Circovirus type 2 Antibody ELISA Test Kit for Pig 192 Wells/Kit GMP

1. Porcine Test Kit Usage

GreenSpring® Porcine Circovirus (PCV2) ELISA Test Kit is used to detect Porcine Circovirus type 2 antibodies in swine serum; pig farm immune status assessment of Porcine Circovirus and Porcine Circovirus epidemiological investigation. Although the results cannot distinguish between immune and infectious antibodies, determining whether it is vaccine antibodies or wild virus infection requires a combination of clinical data, immune status, and other diagnostic methods.

2. Porcine Test Kit Principle

GreenSpring® PCV2 Antibody ELISA Detection Kits are made from antigen-coated microtiter plates (PCV antigen-coated) and other reagents. It applies the principle of solid-phase ELISA to PCV-Ab in serum, and then adds enzyme conjugate to specifically bind to the complex of coated antigen + PCV-Ab + enzyme-labeled anti-pig IgG antibody on the microplate. With TMB substrate, it produces a certain amount of color. The depth of color is related to the content of PCV-Ab. When the color value is greater than the critical value, the pigs are well inoculated or have natural infection.

3. PCV Type 2 Ab ELISA Test Kit Components

4. Materials Required But Not Provided

1) Microplate Reader (double-wave length: 450/630 nm).

2) Precise micropipette (single-channel 1-100ul,0.5-10ul,multi-channel 30-300ul)

3) Constant temperature box or water bath box.

4) Oscillator.

5) Microplate Washer.

6) Disposable tips (10ul, 200ul)

7) Deionized water

5. Sample requirement

1) The sample is pig serum, which should be free of bacteria when collected. Store at 2-8°C for no more than 1 week, and at -20°C for long-term storage.

2) Avoid using samples that are severely hemolyzed, precipitated, contaminated with bacteria or protein suspensions.

3) Anticoagulants such as EDTA and heparin sodium did not affect the results.

6. Preparation

1) Leave the ELISA reagents at room temperature (20-25°C) for 30 minutes for best results.

2) Sample dilution: Dilute the sample 40 times with sample diluent. (The color will change after adding the solution), the diluted sample needs to be mixed well for better results.

3) Preparation of washing solution: Dilute 20× concentrated washing solution 20 times with deionized water.

7. Procedure

1) Take out the coated plates (Can be detached) and record the sample position on a worksheet. Set 2 wells for negative control serum, add undiluted negative control serum, 2 wells for positive control serum, add undiluted positive control serum, 100μL/well. Others are sample wells, add the diluted sample, 100μL each(both single- well and double-well test is OK).

2) Mix gently, cover and incubate at 37℃ for 30 min.

3) Remove adhesive foil. Pour the liquid out of the wells, add the diluted Washing buffer into each well fully, directly pour out, no need to be static. Repeat 3 times, at last time pat to dry on absorbent paper.

4) Add 100μL enzyme conjugate into each well.

5) Cover plate with new adhesive foil. Incubate at 37 ℃ for 30 min.

6) Repeat step 3(washing).

7) Add substrate 100ul into each well, mix properly, incubate for 10 min at 37 ℃ in the dark.

8) Add stop solution 50μL into each well, mix gently and determine the result.

9) Measure the OD value of each well with a photometer at dual-wave length 450nm/630nm.

Specifications: 96 wells×2.

Expiry date: 12 months.

Storage: Store at 2~8℃, in the dark.

Production Date: On outer-packing of the test kit.

Q1: How long will it take for you to ship?
A1: Usually ships within 7 working days.

Q2: Do you support OEM/ODM?
A2: can be supported. We can customize according to your specific needs and specific quantities.

Q3: How is your factory doing in terms of quality control?
A3: We have ISO9001 certification and ISO13485 certification, so far all the production process, we have standard rules, we comply with the relevant behavior and laws of the government.

Q4: Is after-sales service guaranteed?
A4: We provide professional online technical after-sales service. If the product fails during the experiment, we can provide
one-to-one guidance through telephone, video and other forms.

Q5: What is your minimum order quantity?
A5: 1Kit.

Q6: What is the shipping method?
A6: Usually by air, we will choose the best shipping method for you, and we can also ship according to the shipping method you require.