QPCR Hotstart Taq DNA Polymerase

For research use only

Components

[1] qPCR Buffer contains Mg²⁺ and other components that are necessary for qPCR.

Storage

This reagent should be kept at -20°C. The shelf life is 2 years when stored properly.

Description

QPCR Hotstart Taq DNA Polymerase is a hot-start Taq DNA polymerase modified by antibody-like technology, and the activity is wholly inhibited at room temperature, which results in less non-specific amplifications. Depending on high temperature to activate the enzyme activity, the reaction has very high specificity and sensitivity. QPCR Hotstart Taq DNA Polymerase adopts advanced production technology, does not introduce contamination of animal-derived DNA. The enzyme has stronger stability, and incomparable advantages over antibody-modified hot-start Taq DNA polymerase. The pre-denaturation time is reduced to 3 minutes, and the amplification efficiency is higher than that of most chemical-modified hot-start DNA polymerase. The elongation rate is 1kb/min. It is a special polymerase for real-time quantitative PCR (qPCR), both dye method and probe method.

Unit Definition

Using activated salmon sperm DNA as template/primer, the activity of ingesting 10 nmol total nucleotides as acid insoluble substances within 30 min at 74°C is defined as 1 unit of activity (U).

Storage Buffer

200mM Tris-HCl, 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 50% (V/V) glycerol, 0.5% Triton X -100

10× qPCR Buffer with Mg²⁺

50mM KCl, 100mM Tris-HCl, 200mM NH₄Cl, 20mM MgCl₂

Applications

High-specificity amplification & multiplex qPCR: hot-start polymerase with antibody-like modification

Thermostable: half-life over 40 min at 95°C incubation

Generates 3'-dA overhangs PCR products

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.