DSPath 4X RNA RT PCR One Step RT QPCR Master Mix V5005/V5006
DSPathTM 4X One-Step Multiplex Master Mix
DSPath 4X One-Step Multiplex Master QPCR Mix V5005 (200rxn 20μL/Rxn)
Cat. No.: V5005, V5006
Components
| Component | V5005 (200 rxn, 20 μl/rxn) | V5006 (5000 rxn, 20 μl/rxn) |
| DSPathTM 4X Multiplex Master Mix * | 1 ml × 1 | 25 ml × 1 |
* Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase, dNTPs including dUTP and buffer components.
Storage
This reagent should be kept at -15~-25°C.
Description
The DSPathTM 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.
Product Selection Guide
| Cat. No. | V5001/V5002 | V5005/V5006 | V5005L/V5006L | V5007/V5008 | V5007L/V5008L | V5009/V5010 | V5011 |
| Product Name | One-step Probe RT-qPCR Kit | DSPathTM4X One-Step Multiplex Master Mix | DSPathTM4X One-Step Multiplex Master Mix (Lyophilized) | 2X One Step Prime RT-qPCR Mix | 2X One Step Prime RT-qPCR Mix (Lyophilized) | One-step Probe RT-qPCR Kit V2 | One-step Probe RT-qPCR Kit V3 |
| Mixture Format | Enzyme Mix+2X Buffer Mix | 4X All-in Mix | 4X All-in Lyophilized Powder | 2X All-in Mix | 2X All-in Lyophilized Powder | Enzyme Mix+2X Buffer Mix | Enzyme Mix+5X Buffer Mix |
| Mode/Time | Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~120 min | Fast/~60 min or Standard/~88 min | Fast/~45min or Standard/~80min |
| RT Temperature | 50~55°C | 48~55°C | 48~55°C | 48~55°C | 48~55°C | 50~55°C | 50~55°C |
| PCR Enzyme | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq | Antibody-modified hotstart Taq |
| Heat-labile UDG | + | + | + | + | + | + | + |
| Sensitivity | Very high | Very high | Very high | Very high | Very high | Ultra high | Ultra high |
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
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