General RNA Extraction Kit

Catalog No.: R1051 (50 preps)

Kit Contents

Materials supplied by the users:

• Chloroform

• Ethanol (96–100%)

Description

General RNA Extraction Kit provides a simple method of isolating total RNA from a wide range of sample types and amounts. In general, samples are lysed and then homogenized in the presence of guanidinium isothiocyanate, a chaotropic salt which is capable of protecting the RNA from endogenous RNases. After homogenization, ethanol is added to the sample. The sample is then processed through a spin column containing a clear silica-based membrane where the RNA binds. Any impurities are effectively removed by subsequent washing. The purified total RNA is then eluted in RNase-Free Water and is suitable for use in a variety of downstream applications.

Downstream Applications

Purified RNA is free of impurities and enzyme inhibitors, and have an A_260/280=1.8-2.0, is suitable for applications such as:

• Reverse transcription PCR (RT-PCR)

• Real-time quantitative PCR (qPCR)

• Northern blotting

• Nuclease protection assays

• RNA amplification for microarray analysis

• cDNA library preparation after poly(A)+ selection

Features

• Stable yield

• Reliable performance of high-quality purified total RNA in downstream applications

Storage

DEPC-treated water store at -20 ̊C, other reagents store at 2-8 ̊C, protect from light. Kit contents are stable for up to 12 months when stored properly.

GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.