Components

Storage

This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.

Description

SYBR® Green Blue qPCR Mix is a 2X concentrated real-time quantitative PCR premix solution that is mixed with a blue sample loading indicator and a universal ROX. It does not require adjusting the ROX concentration for different instruments, making it easy to operate. The innovative hot start mechanism can reduce the interference of primer dimer and other secondary products on the reaction, significantly improve the specificity and amplification efficiency of quantitative PCR, and obtain a wider range of quantitative amplification. Using a new enhancer, the fluctuation of PCR efficiency for various target fragments can be controlled within a minimum range, and repeated freeze-thaw has little effect on the amplification performance. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Contains sample adding indicator to reduce sample adding errors

• Compatible with many Real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• Contains universal ROX reference dye

Testing Data:
Power Green qPCR Mix Part Number: P2101/P2102
The Power Green qPCR Mix utilizes an antibody-modified hot-start Taq DNA polymerase to enhance the specificity of the reaction, ensuring the accuracy of gene quantification analysis. Specially optimized, this product allows for the preparation of the reaction system without the need for an ice box, and can be directly prepared at room temperature with components such as PCR Mix, primers, and templates. The prepared PCR reaction system can be kept at room temperature for 24 hours without changing the amplification efficiency, ensuring the stability of the workbench for large-scale testing.

qPCR Amplification Curve (left) and Melting Curve (right)

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.