SuperⅡ Hotstart Taq Polymerase

#P1211, 250U

Concentration: 5U/μl

Contents:

Store at -20°C

For research use only.

In total 2 vials.

Description

SuperⅡ Hotstart Taq Polymerase is a dual-antibody modified hot-start polymerase with ultra-low nucleic acid background and high purity, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited completely at room temperature. Its initial-denaturation time can be reduced to 1 minute. SuperⅡ Hotstart Taq provides excellent specificity, sensitivity and yield. The reaction system can be prepared at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 2min/kb (simple template up to 20s/kb) separately. SuperⅡ Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The products of SuperⅡ Hotstart Taq have overhanged dA at 3’-end.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Applications

• High-specificity amplification & multiplex PCR

• IVD product development and testing

Features

• Thermostable: half-life over 40 min at 95°C incubation

• Generates 3'-dA overhangs PCR products