Taq Antibody #P9131 #P9132
Cat. No./Spec.: P9131/500 U; P9132/5,000 U
Concentration: 10 U/µL
Description
Taq Antibody is a monoclonal antibody against Taq DNA polymerase used in Hot Start PCR that binds to Taq to inhibit DNA polymerase activity and effectively inhibit nonspecific annealing of primers and nonspecific amplification induced by primer dimers. Anti-Taq antibodies denaturate during the initial DNA denaturation step of the PCR reaction, so no special anti-Taq antibody inactivation treatment is required for the use of this product. The Taq enzyme can be used under conventional PCR conditions.
Components
| Component | P9131(500 U) | P9132(5,000 U) |
| Taq Antibody (10 U/µL) | 50 µL | 500 µL |
Storage
This reagent should be kept at -20°C.
Unit Definition
Mix Taq Antibody with Taq DNA Polymerase, warm bath for 10 min at 25°C. The amount of Taq Antibody that inhibits more than 90% of the activity of Taq DNA Polymerase of 1 U for 10 min at 55°C is defined as 1 U.
Application
It is suitable for hot-start PCR and quantitative PCR reactions based on Taq DNA polymerase.
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