Components

Storage

This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.

Description

Super SYBR^® Green qPCR Mix is a 2X concentrated real-time PCR master mix that requires only the addition of template and primers to perform the reaction. The use of an innovative hotstart mechanism can reduce the interference of primer-dimers and other secondary products on the reaction, which can significantly improve the specificity and amplification efficiency of quantitative PCR, and obtain a wider quantitative amplification region. The new enhancer minimizes fluctuations in PCR efficiency for various target fragments, and repeated freeze-thaw cycles have minimal impact on amplification performance. This product comes with a special ROX Reference Dye, which is suitable for all qPCR instruments, eliminating the need to adjust the concentration of ROX on different instruments.

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

Features

• Compatible with common Real-time systems without the need to adjust ROX reference dye

• Hot-start technology brings high specificity and reproducible amplification

Testing Data:
Power Green qPCR Mix Part Number: P2101/P2102
The Power Green qPCR Mix utilizes an antibody-modified hot-start Taq DNA polymerase to enhance the specificity of the reaction, ensuring the accuracy of gene quantification analysis. Specially optimized, this product allows for the preparation of the reaction system without the need for an ice box, and can be directly prepared at room temperature with components such as PCR Mix, primers, and templates. The prepared PCR reaction system can be kept at room temperature for 24 hours without changing the amplification efficiency, ensuring the stability of the workbench for large-scale testing.

qPCR Amplification Curve (left) and Melting Curve (right)

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.