Taq DNA Ligase

Cat. No./Spec.: E1012-A/1,000 U; E1012-B/2,000 U; E1012-C/10,000 U
Concentration: 40 U/µL

Product Description
Taq DNA Ligase is a heat-resistant ligase that catalyzes the formation of phosphodiester bonds at the 5´ -phosphate and 3´-hydroxyl ends of two adjacent DNA strands. This reaction can occur only when the two oligonucleotide chains are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotide chains. Therefore, it can be used to detect single nucleotide variants. Taq DNA ligase uses NAD as a cofactor. Taq DNA ligase was active in the range of 37-75°C.

Storage Condition & Shelf Life
Store at -20°C. The product is valid for 2 years.

Source
Recombinant E. coli strain containing ligase gene cloned from Thermus aquaticus HB8.

Unit Definition
1 unit is the amount of enzyme required to bind 50% of 1 µg BstEII enzyme to the 12-base pair sticky end of λ DNA for 15 minutes at 45 ° C at a total reaction volume of 50 µl.

Scope of Application
• Using ligase chain reaction (LCR) and ligase detection reaction (LDR) to specifically detect alleles
• Incorporating phosphorylated oligonucleotides during primer extension amplification for mutation detection