General Reagents T4 Beta-glucosyltransferase
Price:
USD 207-230/500 U
MOQ:
500 U
Delivery Time:
8 work days
Brand:
GDSBio
Product Description
T4 β-glucosyltransferase
Cat. No./Spec.: E1020-A/500 U
Concentration: 5 U/µL
Product Description
T4 β-glucosyltransferase (T4 BGT) is capable of transferring the glucosyl group from uridine diphosphate glucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues within double-stranded DNA, resulting in the formation of β
-glucosyl-5-hydroxymethylcytosine. This enzyme is formulated for rapid reaction times without compromising the efficiency of the reaction. It can glucosylate 5-hmC on 1 μg of DNA in 15 minutes at 37°C.
Cat. No./Spec.: E1020-A/500 U
Concentration: 5 U/µL
Product Description
T4 β-glucosyltransferase (T4 BGT) is capable of transferring the glucosyl group from uridine diphosphate glucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues within double-stranded DNA, resulting in the formation of β
-glucosyl-5-hydroxymethylcytosine. This enzyme is formulated for rapid reaction times without compromising the efficiency of the reaction. It can glucosylate 5-hmC on 1 μg of DNA in 15 minutes at 37°C.
Storage Condition & Shelf Life
Store at -20°C.
Unit Definition
A unit is defined as the amount of enzyme required to protect 0.5 μg of fully 5-hydroxymethylated 1095bp PCR fragment from digestion by MunI within 1 hour at 37°C in a 50 μL volume of the recommended reaction buffer.
Features
- Specificity - Selectively transfers glucose to the hydroxymethyl group of 5-hmC.
- Speed - Completes the glucosylation of 1 μg DNA in 15 minutes.
- Convenience - Comes with optimized buffer and UDP-glucose included.
Scope of Application
- Site-specific detection of 5-hmC.
- Enrichment of DNA containing 5-hmC.
- Labeling of 5-hmC residues using radioactive UDP-glucose donor.
Store at -20°C.
Unit Definition
A unit is defined as the amount of enzyme required to protect 0.5 μg of fully 5-hydroxymethylated 1095bp PCR fragment from digestion by MunI within 1 hour at 37°C in a 50 μL volume of the recommended reaction buffer.
Features
- Specificity - Selectively transfers glucose to the hydroxymethyl group of 5-hmC.
- Speed - Completes the glucosylation of 1 μg DNA in 15 minutes.
- Convenience - Comes with optimized buffer and UDP-glucose included.
Scope of Application
- Site-specific detection of 5-hmC.
- Enrichment of DNA containing 5-hmC.
- Labeling of 5-hmC residues using radioactive UDP-glucose donor.
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Company
Guangzhou Dongsheng Biotech Co., Ltd
Location
Room 305, Building A, No. 179, Guangpu East Road, Huangpu District, Guangzhou, Guangdong