Fast Restriction Endonuclease BglII
Price:
USD 93.6-104/100 rxns
MOQ:
100 rxns
Delivery Time:
8 work days
Brand:
GDSBio
Product Description
BglⅡ
Cat. No./Spec.: E1023-A/100 rxns
Recognition site:
5' A ↓ G A T C T 3'
3' T C C A G ↑ A 5'
Product Description
The BglⅡ restriction endonuclease recognizes the A^GATCT site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
Cat. No./Spec.: E1023-A/100 rxns
Recognition site:
5' A ↓ G A T C T 3'
3' T C C A G ↑ A 5'
Product Description
The BglⅡ restriction endonuclease recognizes the A^GATCT site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
Storage Condition & Shelf Life
Store at -20°C.
Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.
Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis
Store at -20°C.
Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.
Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis
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Company
Guangzhou Dongsheng Biotech Co., Ltd
Location
Room 305, Building A, No. 179, Guangpu East Road, Huangpu District, Guangzhou, Guangdong