500bp Ladder

Concentration: 92ng/µl

Components

Recommended Loading

3-5 µl/Lane.

Concentration

Typical Bands 100 ng/5µl

Other Bands 40 ng/5µl

Recommended Electrophoresis Condition

5 µl/Lane, 8 cm 1%Agarose Gel, 1×TAE, 7V/cm, 45min

Contents(bp)

500,1000,1500,2000,2500,3000,3500,4000,4500,5000

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

Description

500bp ladder is ideal for determining the size of double-stranded DNA from 500 to 5,000 base pairs. The ladder consists of 10 linear double-stranded fragments .The 2,500bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 2,000bp are 40ng. The 2,000bp fragment is 100ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR® Green I.

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