NGS Library Construction Fast DNA Library Prep Kit
Fast DNA Library Prep Kit
Instruction for Use
【Product Name】
Fast DNA Library Prep Kit
【Cat. No./Spec.】
K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns
【Product Description】
Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.
【Sample Type】
| Application | Sample Type | Recommended Amount |
| Whole genome sequencing | High quality complex genomes | 50ng-1μg |
| Target capture sequencing of whole exome | High quality complex genomes | 10ng-1μg |
| Target capture sequencing of whole genome | FFPE DNA | ≥50ng |
| Target capture sequencing of whole genome | cfDNA/ctDNA | ≥100pg |
| Whole genome sequencing | Microbial genome | 1ng-1μg |
| Whole genome sequencing(PCR-free) | High quality DNA |
≥50ng (no size selection) ≥200ng (size selection) |
| ChIP-Seq | ChIP DNA | ≥100pg |
| Targeted sequencing | Amplicon | ≥100pg |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.
【Components】
| Component | 24 rxns | 96 rxns |
| End Repair & A-Tailing Mix | 480 μl | 4×480 μl |
| Fast DNA Ligase | 120 μl | 2×240 μl |
| Fast Ligation Buffer | 600 μl | 4×600 μl |
| 2× HIFI Library PCR Master Mix | 600 μl | 4×600 μl |
| Primer Mix* | 120 μl | 480 μl |
* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.
Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.
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