FS PCR Master Mix PCR Kit For DNA RNA Nucleic Acid Detection P3072 1ml×5

FS^TM Kit

#P3072, 5 ml

Contents:

Description

FS^TM Kit contains FS^TM Taq DNA Polymerase and a premixed, ready-to-use FS^TM Reaction Mix, which contains dNTPs, Mg²⁺ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction Mix has higher amplification sensitivity due to optimized components and enhancer.

FS^TM Taq DNA Polymerase is the latest generation Taq-based DNA polymerase developed by GDSBio. It possesses high amplification efficiency as Taq DNA Polymerase does, and fast elongation ability as KOD polymerase does, can be used in various kinds of PCR. The FS^TM PCR Buffer is designed for FS^TM Taq DNA Polymerase, can be used in fast amplification reaction. The elongation rate of FS^TM Taq DNA Polymerase can be as fast as 3kb/min. It can shorten the amplification time by 2/3. It has 5' to 3' polymerase activity, but lacks of 3' to 5' exonuclease activity, that results in a 3'-dA overhangs PCR product.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH8.0), 1mM DTT, 0.1mM EDTA, 3.2mM MgCl_2, 100mM KCl, 0.1% Triton X-100, 0.1% TW 20, 0.2mg/ml BSA, 50% Glycerol

Composition of the 2× FS^TM Reaction Mix

2× FS^TM Buffer, 0.4mM dNTPs, 3.2mM MgSO_4, 0.02% bromophenol blue.

FS^TM Reaction Mix is a proprietary formulation optimized for robust performance in PCR.

Applications

• High throughput PCR

• Long and complex template PCR

• Routine PCR with high reproducibility

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added

when prepare final PCR

• High yields of PCR products with minimal optimization

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

• Higher sensitivity and fast compared to conventional Taq DNA Polymerase

• Flexible: the amount of polymerase is flexible and controllable.

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.