Taq Plus DNA Polymerase

Description

Taq Plus DNA Polymerase is a mixture of Taq and Pfu polymerase, blends the processivity of taq with the high fidelity of Pfu. The two enzymes act synergistically during PCR to generate more accurate and longer PCR products with greater yields compared to Taq DNA Polymerase alone. The elongation rate is 3kb/min. It can amplify DNA target up to 20 kb (simple template). And it is suitable as a direct replacement for ordinary Taq Polymerase in most applications. PCR products amplified by Taq Plus are mixture of blunt-ends and 3' dA-overhangs. The error rate of this PCR amplification is 1.0×10^-5 per nucleotide per cycle.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH 8.0), 100mM KCl, 3mM MgCl_2, 1mM DTT, 0.1% NP-40, 0.1% Tween20, 0.2mg/ml BSA, 50% (V/V) glycerol.

10× PCR Buffer without Mg²⁺

100mM Tris-HCl (pH 8.8), 500mM KCl, 1% Triton X-100.

Applications

• Amplification of long template up to 20 kb

• Amplification of complex template

• High fidelity PCR

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.