Pfu Kit

#P3022, 5 ml

Pfu Kit For Nucleic Acid PCR Detection P3022 1ml×5

Contents:

Store at -20°C

For research use only.

In total 6 vials.

Description

Pfu Kit contains Pfu DNA Polymerase and a premixed, ready-to-use Pfu Reaction Mix, which contains dNTPs, Mg²⁺ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction mix contributes to high specificity by optimizing the system, reducing primer-dimer rate. PCR amplification sensitivity is controllable due to the amount of DNA polymerase is flexible.

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA Polymerase, highly thermostable Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis. The elongation velocity is 1kb/min (70~75°C)

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% NP-40, 0.5% TW 20, 50% Glycerol

Composition of the 2× Pfu Reaction Mix

2× Pfu Buffer, 0.4mM dNTPs, 3.2mM MgSO_4, 0.02% bromophenol blue.

Pfu Reaction Mix is a proprietary formulation optimized for robust performance in PCR.

Applications

• High fidelity PCR

• Routine PCR with high reproducibility

• Site-directed mutagenesis

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added when prepare final PCR

• High yields of PCR products with minimal optimization

• Fast: saves time due to reduced number of pipetting steps.

• Reproducible: lower contamination and pipetting error risk

• Flexible: the amount of polymerase is flexible and controllable.

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.