Optimus^TM Hotstart Taq Mix

#P2041, 1 ml

Contents:

Store at -20°C

For research use only.

In total 2 vials.

Description

2× Optimus^TM Hotstart Taq Mix is a premixed, ready-to-use solution containing Hotstart Taq DNA Polymerase, dNTPs, Mg²⁺ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Hotstart Taq DNA Polymerase.

Optimus^TM Hotstart Taq DNA Polymerase is a hot-start polymerase with antibody-like modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 1min/kb (simple template up to 20s/kb) separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The product of Hotstart Taq has overhanged dA at 3’-end. Optimus^TM Hotstart Taq DNA Polymerase has zero animal source pollution by being produced with advanced antibody-like modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerases and the initial-denaturation time can be reduced to 3 minutes.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Composition of the 2× Optimus^TM Hotstart Taq Mix

0.5U/ul Optimus^TM Hotstart Taq DNA Polymerase, 2× PCR buffer, 0.4mM dNTPs, 3.2mM MgCl₂, 0.02% bromophenol blue.

Applications

• High-specificity amplification & multiplex PCR: hot-start polymerase with antibody-like modification

• Thermostable: half-life over 40 min at 95°C incubation

• Generates 3'-dA overhangs PCR products

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.