Optimus^TM Hotstart Taq DNA Polymerase

#P1042, 1,000U

Concentration: 5U/μl

Contents:

Store at -20°C

For research use only.

In total 4 vials.

Description

Optimus^TM Hotstart Taq DNA Polymerase is a hot-start polymerase with chemical modification, which brings higher specificity by reducing non-specific products as the enzyme activity is temperature-dependent and is inhibited at room temperature. The amplification length and speed can reach to 5 kb (simple template) and 1min/kb (simple template up to 20s/kb) separately. Hotstart Taq has 5’-3’ polymerase activity, but no 3’-5’ exonuclease activity. The product of Hotstart Taq has overhanged dA at 3’-end. Optimus^TM Hotstart Taq DNA Polymerase has zero animal source pollution by being produced with advanced chemical modification. And it is much more stable than antibody-modified hot-start polymerase. Its efficiency is higher than most chemical-modified polymerase and the initial-denaturation time can be reduced to 3 minutes.

Optimus^TM Hotstart Taq DNA polymerase is an innovative and useful product.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

200mM Tris-HCl, 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% Tween20, 50% (V/V) glycerol, 0.5% Triton X -100

10× Hotstart Buffer with Mg2+

50mM KCl, 100mM Tris-HCl, 200mM NH₄Cl, 20mM MgCl₂.

Applications

• High-specificity amplification & multiplex PCR: hot-start polymerase with chemical modification

• Thermostable: half-life over 40 min at 95°C incubation

• Generates 3'-dA overhangs PCR products

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.