Long Taq Kit

#P3062, 5 ml

Contents:

Store at -20°C

For research use only.

In total 9 vials.

Description

Long Taq Kit contains Long Taq DNA Polymerase and a premixed, ready-to-use Long Reaction Mix, which contains dNTPs, Mg²⁺ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. Long Taq Kit contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer. Using the kit in your PCR reaction results in a mix of blunt-end and 3'-dA overhangs PCR products.

Long Taq DNA Polymerase, a combination of two thermostable DNA polymerases, Taq and Pfu, is a special formulation designed for amplifying large fragments. This specially formulated Long Taq was shown to amplify long templates from λ phage genome that up to 20 kb. The elongation rate is 3kb/min. It is also a better choice for amplifying complex template, such as GC-rich template. Long Taq is suitable as a direct replacement for ordinary Taq Polymerase in most applications. Using Long Taq in your PCR reactions results in 3´-dA overhangs PCR products, which can be used in TA clone.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% NP-40, 0.5% TW 20, 50% (V/V) Glycerol

Composition of the 2× Long Reaction Mix

2× Long PCR Buffer, 0.4mM dNTPs, 3.2mM MgCl_2, 0.02% bromophenol blue.

Long Reaction Mix is a proprietary formulation optimized for robust performance in PCR.

Applications

• PCR for long templates of up to 20 kb

• High reproducible, high throughput PCR for complex template

Features

• Convenient: only primers and template DNA are added

when prepare final PCR

• Longer fragment: amplify long templates as long as 20 kb

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

• Amplification of complex template (GC-rich or repetitive sequence)

• Flexible: the amount of polymerase is flexible and controllable.

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.