PCR Kit

#P3012, 5 ml

Contents:

Store at -20°C

For research use only.

In total 6 vials.

Description

PCR Kit contains Taq DNA Polymerase and a premixed, ready-to-use PCR Reaction Mix, which contains dNTPs, Mg²⁺ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. PCR Kit contributes to highly reproducible PCR by reducing the risk of pipetting errors, miscalculation and contamination. It also contributes to higher sensitivity by adding enhancer. Using the kit in your PCR reaction results in 3’-dA overhangs PCR products.

Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH8.0), 1mM DTT, 0.1mM EDTA, 3.2mM MgCl_2, 100mM KCl, 0.1% Triton X-100, 0.1% TW 20, 0.2mg/ml BSA, 50% Glycerol.

Composition of the 2× PCR Reaction Mix

2× PCR Buffer, 0.4mM dNTPs, 3.2mM MgSO_4, 0.02% bromophenol blue.

PCR Reaction Mix is a proprietary formulation optimized for robust performance in PCR.

Applications

• High throughput PCR

• Routine PCR with high reproducibility

• Generation of PCR products for TA cloning

Features

• Convenient: only primers and template DNA are added

when prepare final PCR

• High yields of PCR products with minimal optimization

• High efficiency: saving your time by simplifying the process

• Reproducible: lower contamination and pipetting error risk

• Flexible: the amount of polymerase is flexible and controllable.

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.