PCR Sample Preparation Solution

PCR Sample Preparation Solution P9051 P9052

#P9051, 50 preps

#P9052, 250 preps

Components

Store at RT

For research use only.

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests.

Protocol

1. Sample Preparation
a. For tissue
Add 3-5mg of sample tissue to a 1.5ml microcentrifuge tube.
b. For hair
Take take two pieces of hair with follicle. Cut the hair root with follicle into a 1.5ml microcentrifuge tube.

c. For buccal swab

Ensure that person providing the sample has not consumed any food or drink in the 30 minutes prior to the sample collection.

Wipe the buccal inner wall 10 times with aseptic cotton swab. Cut off swab into a 1.5ml microcentrifuge tube.

Add 800 μl TE and mix thoroughly by vortexing. Transfer 500 μl of the swab solution to a new microcentrifuge tube.

Centrifuge for 3 min at 5,000 rpm. Discard the flow-through.

d. For culture cells

For resuspend cells, collect 1 ×10³-10⁴ cells to a 50 μl PCR system.

For adherent cells, Add 3-5mg cells to a 1.5ml microcentrifuge tube.

e. For serum, DNA virus

2. Add 180 μl Extracion Solution(90 μl for buccal swab). Mix thoroughly by vortexing. Incubate for 10 minutes at 95 ℃.

3. Add 20 μl Neutralization Solution(10 μl for buccal swab).Mix thoroughly by vortexing. Centrifuge for 2min at 5,000 rpm.

4. Use 0.5-2 μl flow-through for PCR amplifica

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.