RNasin (RNase Inhibitor) #R2011 1,000U

RNasin (RNase Inhibitor)

Components

Store at -20°C

For research only

In total 1 vial.

Description

RNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms.

Source

E.coli cells with a cloned gene encoding mammalian ribonuclease inhibitor.

Storage Buffer

20mM HEPES-NaOH (pH7.5), 50mM NaCl, 8mM DTT, 0.5mM ELUGENT Detergent and 50% (v/v) glycerol.

Feature

Free of endodeoxyribonucleases, exodeoxyribonucleases, rib- onucleases and phosphatases.

Definition of Activity Unit
One unit of the protein inhibits the activity of 5ng RNase A by 50%.
Inhibitor activity is assayed in the following mixture: 100 mM Tris-HCl (pH 7.5), 1.2 mM EDTA, 0.1 mg/ml BSA, 100 ng/ml RNase A, 0.1 mg/ml E.coli [3H]-RNA, 50 mg/ml yeast RNA, 8 mM DTT.

Applications

• Inhibition of RNA degradation in the following procedures:
-in vitro transcription;

-cDNA synthesis;
-in vitro translation;
-isolation of mammalian cell fractions that contain mRNA

protein complex.
• Separation and identification of specific ribonuclease activities.

• Studies of tumor suppression.

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