Heat Labile UDG

For research use only

Components

Storage

This reagent should be kept at -20°C.

Description

Heat Labile UDG is purified from the expression product of an recombinant E. coli strain containing the UDG gene cloned from cold-adaptive marine bacteria (psychrophilic Marine bacterium). UDG (Uracil-DNA Glycosylase) catalyzes the hydrolysis of uracil bases of the single or double stranded DNA containing dU and the N-glycoside bonds of the glycosphosphoric acid skeleton to release free uracil. The resulting base-free site is easily fractured by hydrolysis. This product is sensitive to high temperature, and above 50°C can make the enzyme irreversible inactivation, suitable for PCR, qPCR, RT-PCR, RT-qPCR system.

Storage Buffer

20 mM Tris-HCl, pH 7.5 at 25°C

0.1 mM EDTA

100 mM KCl

1 mM DTT

Glycerol 50% (v/v)

CA630 0.5% (v/v)

0.5% Tween - 20 (v/v)

Source

Psychrophilic Marine Bacteium derived Heat Labile UDG expressed and purified in E. coli.

Unit Definition

In 70 mM Tris-HCl, pH 7.5, 10 mM NaCl, 1 mM EDTA, 100 g/ml BSA reaction solution, the amount of enzyme required to release 1 nmol uracil from DNA containing dU within 1 h at 37°C was defined as 1 unit of activity (U).

Thermal inactivation

95°C, 2 min

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