2X 1ml NGS Library Construction Multiplex PCR Master Mix
NGS Multiplex PCR Master Mix II, 2X
The NGS Multiplex PCR Master Mix II contains all of the components for NGS multiplex PCR (except for primers and templates) in a single tube, including chemically modified ultra-high fidelity DNA Polymerase, which has 100X amplification fidelity of Taq DNA Polymerase. The PCR products have an ultra-low error rates, and is very suitable for the detection of tumor ctDNA and MRD.
| Cat. No. | Contents | Storage Conditions |
| NM2001 | NGS Multiplex PCR Master Mix II, 2X, 40 reactions |
Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days. The GC Enhancer must be kept at –15°C to –25°C. |
| • NGS Multiplex PCR Master Mix II, 2X (1 × 1 mL) | ||
| • GC Enhancer (1 × 0.25 mL) | ||
| NM2002 | NGS Multiplex PCR Master Mix II, 2X, 400 reactions | |
| • NGS Multiplex PCR Master Mix II, 2X (10 × 1 mL) | ||
| • GC Enhancer (2 × 1 mL) | ||
| NM2003 | NGS Multiplex PCR Master Mix II, 2X, 2000 reactions | |
| • NGS Multiplex PCR Master Mix II, 2X (5 × 10 mL) | ||
| • GC Enhancer (1 × 10 mL) |
[1] The final concentration of each primer in a typical PCR is between 0.05-0.4 μM. In most cases, a final concentration of 0.15 μM gives satisfactory results. Increasing the primer concentration up to 0.4 μM may increase the yield.
[2] Use GC Enhancer only when high GC content targets cannot be amplified under standard conditions.
Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.
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