GDS Fragmentation & End Prep Module K023-A , K023-B
GDS Fragmentation & End Prep Module
【Product Name】
GDS Fragmentation & End Prep Module
【Cat. No./Spec.】
K023-A (24 rxns); K023-B (96 rxns)
【Product Description】
GDS Fragmentation & End Prep Module is designed for library construction of high-throughput sequencing platform. The module combines fragmentation of intact DNA, end repair and dA tailing, reducing operation and reaction time and the amount of template. 100 pg ~ 500 ng DNA template can be efficiently fragmented with 5’ phosphorylated 3' dA-tailed ends. This product contains the enzymes and buffers required for the reaction, easy to use, and compatible with automatic library preparation equipment.
【Components】
| Component | K023-A (24 rxns) | K023-B (96 rxns) |
| GDS FEP Enzyme Mix | 240 μL | 2×480 μL |
| GDS FEP Buffer | 120 μL | 480 μL |
| Neutralization Buffer | 120 μL | 480 μL |
【Storage Condition & Shelf Life】
All reagents should be stored at -20°C. The product is valid for 12 months.
【Application】
Fragmentation, end repair and dA-tailing in dsDNA library construction.
【Protocol】
1. Determine the solvent composition of template DNA, if no EDTA, proceed directly to Step 2; If EDTA is contained, 2.2X magnetic beads should be used for purification, or a corresponding volume of Neutralization Buffer should be added according to the content of EDTA in the following table for Neutralization:
| EDTA Conc. | Volume of Neutralization Buffer |
| 1 mM | 5 μL |
| 0.8 mM | 4 μL |
| 0.6 mM | 3 μL |
| 0.5 mM | 2.5 μL |
| 0.4 mM | 2 μL |
| 0.2 mM | 1 μL |
| 0.1 mM | 0.5 μL |
| <0.1 mM | 0 μL |
2. Ensure that all reagents are completely thawed. Place on ice until use. Prepare the following reaction in a 200 μL PCR tube:
| Reagents | Volume |
| Input DNA | X μL |
| GDS FEP Buffer | 5 μL |
| GDS FEP Enzyme Mix | 10 μL |
| ddH2O | To 50 μL |
3. Vortex the reaction for 5 seconds and briefly spin in a microcentrifuge.
4. In a thermal cycler, with the heated lid set to 105°C, run the following program:
| Temperature | Time |
| 37°C | 5~30 min * |
| 65°C | 30 min |
| 4°C | hold |
* Use the chart below to determine the incubation time required to generate the desired fragment sizes:
| Fragment Size | Time |
| 150 bp | 20-30 min |
| 250 bp | 15-20 min |
| 350 bp | 10-15 min |
| 550 bp | 6-10 min |
- Adapter ligation directly as soon as possible to avoid excessive DNA fragmentation.
【Library Preparation Module】
GDSBio offers the following DNA and RNA library construction modules that can be used in combination for high-quality library preparation:
| Module | Product Name | Cat. No./Spec. |
| cDNA First Strand Synthesis | GDS RNA First Strand Synthesis Module |
K020-A/24 rxns K020-B/96 rxns |
| Directional cDNA Second Strand Synthesis | GDS Directional RNA Second Strand Synthesis Module |
K021-A/24 rxns K021-B/96 rxns |
| Non-Directional cDNA Second Strand Synthesis | GDS Non-Directional RNA Second Strand Synthesis Module |
K022-A/20 rxns K022-B/100 rxns |
| Fragmentation & End Repair | GDS Fragmentation & End Prep Module |
K023-A/24 rxns K023-B/96 rxns |
| Fragmentation | GDS dsDNA Fragmentase |
K024-A/50 rxns K024-B/250 rxns |
| End Repair/dA-Tailing | GDS End Preparation Module |
K025-A/24 rxns K025-B/96 rxns |
| Adapter Ligation | GDS Ligation Module |
K026-A/24 rxns K026-B/96 rxns |
| Amplification | HIFI Library PCR Master Mix |
K007-A/40 rxns K007-B/400 rxns K007-C/2000 rxns |
| Cleanup/Size Selection | GDSPure DNA Selection Magbeads |
NC1011/5 mL NC1012/60 mL NC1013/450 mL |
GDSBio is a high-tech company focused on the development, production and marketing of quality life science products. The company has a complete product line, with PCR technology as the core, focusing on ordinary PCR, fluorescent quantitative PCR, NGS library construction, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular scientific research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.
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