RNase A Solution

BR Grade,For Research Use Only

Cat. No. N9041 Concentration: 10 mg/ml Size: 1 ml

Cat. No. N9042 Concentration: 100 mg/ml Size: 1 ml

Specific Activity: ≥3000 U/mg protein (≥60 Kunitz units/mg protein).

Components

Description
The RNase A is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate.

Applications

--Plasmid and genomic DNA preparation
--Removal of RNA from recombinant protein preparations.
--Ribonuclease protection assays
--Mapping single-base mutations in DNA or RNA

Storage

-20ºC recommended.

Molecular Weight
13.7 kDa monomer.

Definition of Activity Unit
One unit of the enzyme causes an increase in absorbance of 1.0 at 260 nm when yeast RNA is hydrolyzed at 37°C and pH 5.0.
Fifty units are approximately equivalent to 1 Kunitz unit.

Storage Buffer
The enzyme is supplied in: 50 mM Tris-HCl (pH 7.4) and 50% (v/v) glycerol.

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