20bp Ladder

20bp Ladder DNA Marker Electrophoresis M1021 50μg

Concentration: 128ng/µl

Components

Description

20bp ladder is ideal for determining the size of double-stranded DNA from 60 to 300 base pairs in high percentage agarose or non-denaturing polyacrylamide gels. The ladder consists of 13 linear double-stranded fragments. It is a 20 bp step ladder, especially useful for determining 20 bp differences between DNA fragments. The 100bp and 200bp fragments are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 1 ul loading , all fragments except 100bp and 200bp are 8ng, the 100bp and 200bp fragments are 20ng. This ladder is pre-mixed with loading dye and is ready to use.

Recommended Loading

1 µl/Lane in non-denaturing polyacrylamide gel

3-5 µl/Lane in agarose gel

Concentration

Typical Bands, 20ng/µl

Other Bands, 8ng/µl

Recommended Electrophoresis Condition

1 µl/Lane, 20 cm 10% non-denaturing Polyacrylamide Gel, 1×TBE, 8 V/cm, 3h.

Contents (bp)

60,80,100,120,140,160,180,200,220,240,260,280,300.

Storage

Stable for 3 months at RT, for long-time storage, please store at -20 ̊C.

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